
COVID-19 Human IgM IgG ELISAキット(Spike Protein)
マニュアル
フライヤー
製品の主な仕様
測定方法: 間接 ELISA 法
測定波長: 450 nm
測定試料: 血清
交差性: Human
保管温度: HRP 標識抗ヒト IgM 抗体及び HRP 標識抗ヒト IgG 抗体 -20°C保管
上記以外の試薬及び固相化プレート 4°C保管
品質保証期限: 本品の有効期限は製造後 5 カ月です。
プレートへの固相化抗原: SARS-CoV-2 スパイクタンパク(S1 + S2ドメイン, 哺乳類細胞発現系)
キット内容
1. SARS-CoV-2 スパイクタンパク固相化プレート
2. HRP 標識抗ヒト IgM 抗体
3. HRP 標識抗ヒト IgG 抗体
4. 洗浄液(10× PBST)
5. スキムミルク(ブロッキングバッファー用)
6. Diluent 用バッファー (10×)
7. R-1:発色基質(TMB)
8. R-2:反応停止液(1 mol/L 塩酸)
9. マイクロプレートシール
×2
4 μL×1
4 μL×1
50 mL×2
×1
50 mL×1
10 mL×2
10 mL×2
×2
測定原理
-
固相化プレート上の リコンビナント SARS-CoV-2 スパイクタンパク と、これを認識する試料中の抗体(以後、抗スパイクタンパク抗体と略称)を反応させる。
-
反応後、試料を洗浄操作により除去する。
-
固相化プレート上の 抗スパイクタンパク抗体と、HRP (Horseradish peroxidase) 標識抗ヒト IgM / IgG 抗体を反応させる。
-
余剰の HRP 標識抗ヒト IgM / IgG 抗体を洗浄操作により除去する。発色基質を加え、吸光度を測定する。

測定の意義
本キットは、COVID-19 の原因ウイルスである、SARS-CoV-2 のスパイクタンパクを認識するヒトIgM 抗体とヒト IgG 抗体を検出するための研究用 ELISA キットです。
ウイルスの検出には、大きく分けて二種類のアプローチ法があり、PCR 法などのウイルス自体を検出する方法と、感染時に発現する免疫反応(IgG, IgM, IgA)を検出する方法があります。一般的 な感染症において、IgM は感染初期に生成される抗体で、症状の緩和とともに消失します。IgG は IgM に遅れて産生が始まり、治癒後も数カ月にわたって産生され続けます。これにより次に感染し た際の症状が軽くなったり、発症を防いだりすることが知られています。
関連文献
-
Anna Schaffner et al.
Sustained SARS-CoV-2 nucleocapsid antibody levels in nonsevere COVID-19: a population-based study
Clin Chem Lab Med. 2020 Nov. PubMed PMID: 33554502
-
Martin P. Steinbuck et al.
A lymph node–targeted Amphiphile vaccine induces potent cellular and humoral immunity to SARS-CoV-2
Sci Adv. 2021 Feb. PubMed PMID: 33547083
-
Susanne Brandstetter et al.
Symptoms and immunoglobulin development in hospital staff exposed to a SARS‐CoV‐2 outbreak
Pediatr Allergy Immunol. 2020 Oct. PubMed PMID: 32413201
Eur J Clin Microbiol Infect Dis. 2021 Feb. PubMed PMID: 33534090
-
Evangelos Terpos et al.
Anti–SARS-CoV-2 Antibody Responses in Convalescent Plasma Donors Are Increased in Hospitalized Patients; Subanalyses of a Phase 2 Clinical Study
Microorganisms. 2020 Dec. PubMed PMID: 33260775
-
Susanna Scharrer et al.
Humoral response to COVID-19 infection in immunosuppressed patients with inflammatory bowel disease
Eur J Gastroenterol Hepatol. 2021 Jan. PubMed PMID: 33522752
-
Kristin E. Mullins et al.
Validation of COVID-19 serologic tests and large scale screening of asymptomatic healthcare workers
Clin Biochem. 2021 Jan. PubMed PMID: 333472036
-
Larry L. Luchsinger et al.
Serological Assays Estimate Highly Variable SARS-CoV-2 Neutralizing Antibody Activity in Recovered COVID-19 Patients
J Clin Microbiol. 2020 Dec. PubMed PMID: 32917729
-
Dustin D Flannery et al.
Assessment of Maternal and Neonatal Cord Blood SARS-CoV-2 Antibodies and Placental Transfer Ratios
JAMA Pediatr. 2021 Jan. PubMed PMID: 33512440
-
Mario Plebani et al.
Performance of the COVID19SEROSpeed IgM/IgG Rapid Test, an Immunochromatographic Assay for the Diagnosis of SARS-CoV-2 Infection: a Multicenter European Study
J Clin Microbiol. 2021 Jan. PubMed PMID: 33218990
-
Melanie A MacMullan et al.
Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device
J Clin Microbiol. 2021 Jan. PubMed PMID: 33234589
-
Melanie A. MacMullan et al.
ELISA detection of SARS-CoV-2 antibodies in saliva
Sci Rep. 2020 Nov. PubMed PMID: 33257702
-
Mª Isabel Galá et al.
Hospital-Wide SARS-CoV-2 seroprevalence in health care workers in a Spanish teaching hospital
Enferm Infecc Microbiol Clin. 2020 Dec. PubMed PMID: 33485676
-
Tim Meyer et al.
Successful return to professional men’s football (soccer) competition after the COVID-19 shutdown: a cohort study in the German Bundesliga
Br J Sports Med. 2021 Jan. PubMed PMID: 32972979
Clin Microbiol Infect. 2020 Dec 5. PubMed PMID: 33285279
-
N. Davidson et al.
Comparative analysis of three laboratory based serological assays for SARS-CoV-2 in an Australian cohort
Pathology. 2020 Dec. PubMed PMID: 33070955
-
National SARS-CoV-2 Serology Assay Evaluation Group
Performance characteristics of five immunoassays for SARS-CoV-2: a head-to-head benchmark comparison
Lancet Infect Dis. 2020 Dec. PubMed PMID: 32979318
-
Justin Manalac et al.
Evaluation of Abbott anti-SARS-CoV-2 CMIA IgG and Euroimmun ELISA IgG/IgA assays in a clinical lab
Clin Chim Acta. 2020 Nov. PubMed PMID: 32910980
-
Ivana Lapić et al.
The reliability of SARS-CoV-2 IgG antibody testing - a pilot study in asymptomatic health care workers in a Croatian university hospital
Croat Med J. 2020 Dec 31. PubMed PMID: 33410294
-
Nathalie Renard et al.
Performance characteristics of the VIDAS® SARS-COV-2 IgM and IgG serological assays
J Clin Microbiol. 2021 Jan 8. PubMed PMID: 33419947
-
Anja Dörschug et al.
Comparison of Five Serological Assays for the Detection of SARS-CoV-2 Antibodies
Diagnostics (Basel). 2021 Jan 6. PubMed PMID: 33418886
-
Ling L et al.
Analysis of viral load in different specimen types and serum antibody levels of COVID-19 patients
J Transl Med. 2021 Jan 7. PubMed PMID: 33413461
-
Thessicar Antoine-Reid et al.
Cross-Comparison of a Chemiluminescent Platform and a Commercial Receptor Binding Domain-Based ELISA for Detecting SARS-CoV-2 IgG
J Appl Lab Med. 2020 Nov. PubMed PMID: 32750140
-
Julien Marlet et al.
Clinical performance of four immunoassays for antibodies to SARS-CoV-2, including a prospective analysis for the diagnosis of COVID-19 in a real-life routine care setting
J Clin Virol. 2020 Nov. PubMed PMID: 32927357
-
Barnaby Flower et al.
Clinical and laboratory evaluation of SARS-CoV-2 lateral flow assays for use in a national COVID-19 seroprevalence survey
Thorax. 2020 Aug. PubMed PMID: 32796119
-
Adrian Shields et al.
SARS-CoV-2 seroprevalence and asymptomatic viral carriage in healthcare workers: a cross-sectional study
Thorax. 2020 Dec. PubMed PMID: 32917840
-
Christina A Rostad et al.
Quantitative SARS-CoV-2 Serology in Children With Multisystem Inflammatory Syndrome (MIS-C)
Pediatrics. 2020 Dec. PubMed PMID: 32879033
-
Kening Li et al.
Dynamic changes in anti-SARS-CoV-2 antibodies during SARS-CoV-2 infection and recovery from COVID-19
Nat Commun. 2020 Nov 27. PubMed PMID: 33247152
-
Victoria Indenbaum et al.
Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis
PLoS One. 2020 Nov 23. PubMed PMID: 33227020
-
Baweleta Isho et al.
Persistence of serum and saliva antibody responses to SARS-CoV-2 spike antigens in COVID-19 patients
Sci Immunol. 2020 Oct 8. PubMed PMID: 33033173
-
Anges Yadouleton et al.
Limited Specificity of Serologic Tests for SARS-CoV-2 Antibody Detection, Benin
Emerg Infect Dis. 2020 Dec 1. PubMed PMID: 33261717
-
Ricardo Savirón-Cornudella et al.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) universal screening in gravids during labor and delivery
Eur J Obstet Gynecol Reprod Biol. 2021 Jan. PubMed PMID: 33285496
-
Emily Garnett et al.
Clinical Validation and Performance Evaluation of the Automated Vitros Total Anti-SARS-CoV-2 Antibodies Assay for Screening of Serostatus in COVID-19
Am J Clin Pathol. 2020 Nov 4. PubMed PMID: 32864699
-
Lenin Mahimainathan et al.
Evaluation of SARS-CoV-2 Serological Testing in Patients with Multiple Myeloma and Other Hematologic Malignancies on Monoclonal Antibody Therapies
Diagnostics (Basel). 2020 Nov 24. PubMed PMID: 33255154
-
Harvey W Kaufman et al.
Insights from Patterns of SARS-CoV-2 Immunoglobulin G Serology Test Results from a National Clinical Laboratory, United States, March-July 2020
Popul Health Manag. 2020 Nov 19. PubMed PMID: 33216694
-
Martin Risch et al.
Temporal Course of SARS-CoV-2 Antibody Positivity in Patients with COVID-19 following the First Clinical Presentation
Biomed Res Int. 2020. PubMed PMID: 33224987
-
Ainhoa Gutiérrez-Cobos et al.
Evaluation of diagnostic accuracy of 10 serological assays for detection of SARS-CoV-2 antibodies
Eur J Clin Microbiol Infect Dis. 2020 Nov 24. PubMed PMID: 33236268
-
Adrian M Shields et al.
Serological responses to SARS-CoV-2 following non-hospitalised infection: clinical and ethnodemographic features associated with the magnitude of the antibody response
medRxiv. Preprint. 2020 Nov 16. PubMed PMID: 33236029
-
Constant Gillot et al.
An Original ELISA-Based Multiplex Method for the Simultaneous Detection of 5 SARS-CoV-2 IgG Antibodies Directed against Different Antigens
J Clin Med. 2020 Nov 21. PubMed PMID: 33233405
-
Shamez N Ladhani et al.
High prevalence of SARS-CoV-2 antibodies in care homes affected by COVID-19: Prospective cohort study, England
EClinicalMedicine. 2020 Nov 6. PubMed PMID: 33173854
-
Pingping Zhang et al.
Development and evaluation of a serological test for diagnosis of COVID-19 with selected recombinant spike proteins
Eur J Clin Microbiol Infect Dis. 2020 Nov 12. PubMed PMID: 33184753
-
Melkon G DomBourian et al.
Analysis of COVID-19 convalescent plasma for SARS-CoV-2 IgG using two commercial immunoassays
J Immunol Methods. 2020 Nov. PubMed PMID: 32828791
-
Ling Li et al.
Characteristics and serological patterns of COVID-19 convalescent plasma donors: optimal donors and timing of donation
Transfusion. 2020 Aug. PubMed PMID: 32627216
-
Guillaume Beaudoin-Bussières et al.
Decline of Humoral Responses against SARS-CoV-2 Spike in Convalescent Individuals
mBio. 2020 Oct 16. PubMed PMID: 33067385
-
Anita S Iyer et al.
Persistence and decay of human antibody responses to the receptor binding domain of SARS-CoV-2 spike protein in COVID-19 patients
Sci Immunol. 2020 Oct 8. PubMed PMID: 33033172
-
Phuong Nguyen-Contant et al.
S Protein-Reactive IgG and Memory B Cell Production after Human SARS-CoV-2 Infection Includes Broad Reactivity to the S2 Subunit
mBio. 2020 Sep 25. PubMed PMID: 32978311
-
James R. Byrnes et al.
Competitive SARS-CoV-2 Serology Reveals Most Antibodies Targeting the Spike Receptor-Binding Domain Compete for ACE2 Binding
mSphere. 2020 Sep 16. PubMed PMID: 32938700
-
Mohammad Hosein Taziki Balajelini et al.
Using Anti-SARS-CoV-2 IgG and IgM Antibodies to Detect Outpatient Cases with Olfactory and Taste Disorders Suspected as Mild Form of COVID-19: a Retrospective Survey
SN Compr Clin Med. 2020 Nov 3. PubMed PMID: 33169109
-
Tyler J Ripperger et al.
Orthogonal SARS-CoV-2 Serological Assays Enable Surveillance of Low-Prevalence Communities and Reveal Durable Humoral Immunity
Immunity. 2020 Oct 14. PubMed PMID: 33129373
-
Pedro Martínez-Fleta et al.
SARS-CoV-2 Cysteine-like Protease Antibodies Can Be Detected in Serum and Saliva of COVID-19-Seropositive Individuals
J Immunol. 2020 Nov 4. PubMed PMID: 33148714
-
Eshan U Patel et al.
Comparative performance of five commercially available serologic assays to detect antibodies to SARS-CoV-2 and identify individuals with high neutralizing titers
J Clin Microbiol. 2020 Nov 2. PubMed PMID: 33139419
-
Zahra Rikhtegaran Tehrani et al.
Performance of nucleocapsid and spike-based SARS-CoV-2 serologic assays
PLoS One. 2020 Nov 2. PubMed PMID: 33137138
-
Meret Huber et al.
Postinfectious Onset of Myasthenia Gravis in a COVID-19 Patient
Front Neurol. 2020 Oct 6. PubMed PMID: 33123081
-
Ruta Kulkarni et al.
Anti-SARS-CoV-2 IgG antibody response among Indian COVID-19 patients using β-propiolactone-inactivated, whole virus-based indirect ELISA
J Virol Methods. 2021 Jan. PubMed PMID: 33126149
-
Bernd Jahrsdörfer et al.
Independent side-by-side validation and comparison of four serological platforms for SARS-CoV-2 antibody testing
J Infect Dis. 2020 Oct 16. PubMed PMID: 33064789
-
Manuela Pegoraro et al.
Evaluation of three immunochromatographic tests in COVID-19 serologic diagnosis and their clinical usefulness
Eur J Clin Microbiol Infect Dis. 2020 Oct 20. PubMed PMID: 33078222
-
Xiaohui Wang et al.
Accurate Diagnosis of COVID-19 by a Novel Immunogenic Secreted SARS-CoV-2 orf8 Protein
mBio . 2020 Oct 20. PubMed PMID: 33082264
-
Alessandra Sperotto et al.
Screening Procedure for SARS-CoV-2 infection combining Triage, nasopharyngeal swab and serological test in allogeneic stem cell transplantation Recipients Undergoing Outpatient Post Transplant Follow-Up
J Med Virol. 2020 Oct 8. PubMed PMID: 33030745
-
Suliman A Alharbi et al.
Enzyme-Linked Immunosorbent Assay for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) IgM/IgA and IgG Antibodies Among Healthcare Workers
Cureus. 2020 Sep 6. PubMed PMID: 33047077
-
Gláucia Cota et al.
Diagnostic performance of commercially available COVID-19 serology tests in Brazil
Int J Infect Dis. 2020 Oct 9. PubMed PMID: 33039612
-
Massimo Pieri et al.
SARS-CoV-2 infection serology validation of different methods: Usefulness of IgA in the early phase of infection
Clin Chim Acta. 2020 Sep 28. PubMed PMID: 33002475
-
Álvaro A Faccini-Martínez et al.
Serological cross-reactivity using a SARS-CoV-2 ELISA test in acute Zika virus infection, Colombia
Int J Infect Dis. 2020 Sep 28. PubMed PMID: 33002616
-
Nguyen N Nguyen et al.
Correlation of ELISA method with three other automated serological tests for the detection of anti-SARS-CoV-2 antibodies
PLoS One. 2020 Oct 6. PubMed PMID: 33022019
-
Massimo Guerriero et al.
Prevalence of asymptomatic SARS-CoV-2-positive individuals in the general population of northern Italy and evaluation of a diagnostic serological ELISA test: a cross-sectional study protocol
BMJ Open. 2020 Oct 6. PubMed PMID: 33028562
-
Ekasit Kowitdamrong et al.
Antibody responses to SARS-CoV-2 in patients with differing severities of coronavirus disease 2019
PLoS One. 2020 Oct 9. PubMed PMID: 33035234
-
Anja Dörschug et al.
Evaluation of the Xiamen AmonMed Biotechnology rapid diagnostic test COVID-19 IgM/IgG test kit (Colloidal gold)
Eur J Microbiol Immunol (Bp). 2020 Sep 25. PubMed PMID: 32979256
-
Elitza S Theel et al.
Performance Characteristics of Four High-Throughput Immunoassays for Detection of IgG Antibodies against SARS-CoV-2
J Clin Microbiol. 2020 Jul 23. PubMed PMID: 32513859
-
Mei San Tang et al.
Association between SARS-CoV-2 neutralizing antibodies and commercial serological assays
Clin Chem. 2020 Sep 7. PubMed PMID: 32894750
-
Pauline H Herroelen et al.
Humoral Immune Response to SARS-CoV-2
Am J Clin Pathol. 2020 Aug 18. PubMed PMID: 32808976
-
Anna E Merrill et al.
Head-to-Head Comparison of Two SARS-CoV-2 Serology Assays
J Appl Lab Med. 2020 Jul 27. PubMed PMID: 32717056
-
Marzia Nuccetelli et al.
Combined anti-SARS-CoV-2 IgA, IgG, and IgM Detection as a Better Strategy to Prevent Second Infection Spreading Waves
Immunol Invest. 2020 Sep 18. PubMed PMID: 32945214
-
Reuben McGregor et al.
Collaborative networks enable the rapid establishment of serological assays for SARS-CoV-2 during nationwide lockdown in New Zealand
PeerJ . 2020 Sep 3. PubMed PMID: 32953275
-
Suzanne Pickering et al.
Comparative assessment of multiple COVID-19 serological technologies supports continued evaluation of point-of-care lateral flow assays in hospital and community healthcare settings
PLoS Pathog . 2020 Sep 24. PubMed PMID: 32970782
-
Gang Xu et al.
Evaluation of Orthogonal Testing Algorithm for Detection of SARS-CoV-2 IgG Antibodies
Clin Chem. 2020 Sep 7. PubMed PMID: 32894753
-
Diego O. Andrey et al.
Head-to-Head Accuracy Comparison of Three Commercial COVID-19 IgM/IgG Serology Rapid Tests
J Clin Med. 2020 Aug. PubMed PMID: 32722191
-
Diego O. Andrey et al.
Sensitivity, Specificity and Predictive Values of Molecular and Serological Tests for COVID-19: A Longitudinal Study in Emergency Room
Eur J Clin Invest. 2020 Jul 21. PubMed PMID: 32691863
-
Zeno Bisoffi et al.
Diagnostic accuracy of Augurix COVID‐19 IgG serology rapid test
Diagnostics (Basel). 2020 Sep 3. PubMed PMID: 32899333
-
Eshan Patel et al.
Comparative performance of five commercially available serologic assays to detect antibodies to SARS-CoV-2 and identify individuals with high neutralizing titers
medRxiv . 2020 Sep 2. PubMed PMID: 32908987
-
Georg M. N. Behrens et al.
Strategic Anti-SARS-CoV-2 Serology Testing in a Low Prevalence Setting: The COVID-19 Contact (CoCo) Study in Healthcare Professionals
Infect Dis Ther. 2020 Sep 4. PubMed PMID: 32886335
-
Oktavija Đaković Rode et al.
Antibody response and the clinical presentation of patients with COVID-19 in Croatia: the importance of a two-step testing approach
Eur J Clin Microbiol Infect Dis. 2020 Sep 4. PubMed PMID: 32886230
-
Clarence W Chan et al.
Analytical and Clinical Evaluation of the Automated Elecsys Anti–SARS-CoV-2 Antibody Assay on the Roche cobas e602 Analyzer
Am J Clin Pathol. 2020 Aug 20. PubMed PMID: 32814955
-
Fleur Wolff et al.
Monitoring antibody response following SARS-CoV-2 infection: diagnostic efficiency of 4 automated immunoassays
Diagn Microbiol Infect Dis. 2020 Nov. PubMed PMID: 32829098
J Clin Virol. 2020 Aug. PubMed PMID: 32505777
-
Christian Wechselberger et al.
Performance evaluation of serological assays to determine the immunoglobulin status in SARS-CoV-2 infected patients
J Clin Virol. 2020 Oct. PubMed PMID: 32810840
-
Hui-Xia Gao et al.
Detection of serum immunoglobulin M and immunoglobulin G antibodies in 2019 novel coronavirus infected patients from different stages
Chin Med J (Engl). 2020 Jun 20. PubMed PMID: 32221133
-
Sonny Elizaldi et al.
SARS-CoV-2 infection induces robust germinal center CD4 T follicular helper cell responses in rhesus macaques
Res Sq. 2020 Aug 14. PubMed PMID: 32818217
-
Jeffrey D Whitman et al.
Evaluation of SARS-CoV-2 serology assays reveals a range of test performance
Nat Biotechnol. 2020 Aug 27. PubMed PMID: 32855547
-
Verena Haselmann et al.
Results of the first pilot external quality assessment (EQA) scheme for anti-SARS-CoV2-antibody testing
Clin Chem Lab Med . 2020 Aug 27. PubMed PMID: 32853163
-
Maria Infantino et al.
Closing the Serological Gap in the Diagnostic Testing for COVID‐19: The Value of Anti‐SARS‐CoV‐2 IgA Antibodies
J Med Virol. 2020 Aug 13. PubMed PMID: 32790181
-
Verena Haselmann et al.
Comparison of test performance of commercial anti-SARS-CoV-2 immunoassays in serum and plasma samples
Clin Chim Acta. 2020 Nov. PubMed PMID: 32652161
-
G. Caruana et al.
Diagnostic strategies for SARS-CoV-2 infection and interpretation of microbiological results
Clin Microbiol Infect. 2020 Sep. PubMed PMID: 32593741
-
David Peterhoff et al.
A highly specific and sensitive serological assay detects SARS-CoV-2 antibody levels in COVID-19 patients that correlate with neutralization
Infection. 2020 Aug 21. PubMed PMID: 32827125
-
Kasopefoluwa Y Oguntuyo et al.
Quantifying absolute neutralization titers against SARS-CoV-2 by a standardized virus neutralization assay allows for cross-cohort comparisons of COVID-19 sera
medRxiv. 2020 Aug 15. PubMed PMID: 32817961
-
Kathleen M McAndrews et al.
Heterogeneous antibodies against SARS-CoV-2 spike receptor binding domain and nucleocapsid with implications on COVID-19 immunity
JCI Insight. 2020 Aug 14. PubMed PMID: 32796155
-
Aaron J Siegler et al.
Protocol for a national probability survey using home specimen collection methods to assess prevalence and incidence of SARS-CoV-2 infection and antibody response
Ann Epidemiol. 2020 Aug 10. PubMed PMID: 32791199
-
Zahra Rikhtegaran Tehrani et al.
Specificity and Performance of Nucleocapsid and Spike-based SARS-CoV-2 Serologic Assays
medRxiv. 2020 Aug 7. PubMed PMID: 32793933
-
Sebastian Hörber et al.
Evaluation of three fully-automated SARS-CoV-2 antibody assays
Clin Chem Lab Med. 2020 Aug 4. PubMed PMID: 32745068
-
Felipe Pérez-García et al.
Alltest rapid lateral flow immunoassays is reliable in diagnosing SARS-CoV-2 infection from 14 days after symptom onset: A prospective single-center study
J Clin Virol. 2020 Aug. PubMed PMID: 32504945
-
Anita S. Iyer et al.
Dynamics and significance of the antibody response to SARS-CoV-2 infection
medRxiv. Preprint. 2020 Jul 20. PubMed PMID: 32743600
-
Giorgio Caturegli et al.
Clinical Validity of Serum Antibodies to SARS-CoV-2
Ann Intern Med. 2020 Jul 6. PubMed PMID: 32628534
-
Jitendra Prasad Mathuria et al.
Laboratory diagnosis of SARS-CoV-2 - A review of current methods
J Infect Public Health. 2020 Jul. PubMed PMID: 32534946
-
Alexander Krüttgen et al.
Comparison of four new commercial serologic assays for determination of SARS-CoV-2 IgG
J Clin Virol. 2020 Jul. PubMed PMID: 32416599
-
Fatima Amanat et al.
A serological assay to detect SARS-CoV-2 seroconversion in humans
Nat Med. 2020 Jul. PubMed PMID: 32398876
-
Mayara Lisboa Bastos et al.
Diagnostic Accuracy of Serological Tests for covid-19: Systematic Review and Meta-Analysis
BMJ. 2020 Jul. PubMed PMID: 32611558
-
Lisa Sophie Pflüger et al.
Clinical evaluation of five different automated SARS-CoV-2 serology assays in a cohort of hospitalized COVID-19 patients
J Clin Virol. 2020 Jul 17. PubMed PMID: 32763809
-
Corine H. GeurtsvanKessel et al.
An evaluation of COVID-19 serological assays informs future diagnostics and exposure assessment
Nat Commun. 2020 Jul 6. PubMed PMID: 32632160
-
Daniel Shyu et al.
Laboratory Tests for COVID-19: A Review of Peer-Reviewed Publications and Implications for Clinical UIse
Mo Med. 2020 May-Jun. PubMed PMID: 32636542
-
Patrick Sean Sullivan et al.
Detection of SARS-CoV-2 RNA and Antibodies in Diverse Samples: Protocol to Validate the Sufficiency of Provider-Observed, Home-Collected Blood, Saliva, and Oropharyngeal Samples
JMIR Public Health Surveill. 2020 Apr-Jun. PubMed PMID: 32310815
J Clin Virol. 2020 Jun 15. PubMed PMID: 32593133
-
Benjamin Meyer et al.
Validation of a commercially available SARS-CoV-2 serological immunoassay
Clin Microbiol Infect. 2020 Jun 27. PubMed PMID: 3260380
-
Silvia Stringhini et al.
Seroprevalence of anti-SARS-CoV-2 IgG antibodies in Geneva, Switzerland (SEROCoV-POP): a population-based study
Lancet. 2020 Jun 11. PubMed PMID: 32534626
-
Antonio Vittorino Gaddi et al.
The Strategic Alliance between Clinical and Molecular Science in the War against SARS-CoV-2, with the Rapid-Diagnostics Test as an Indispensable Weapon for Front Line Doctors
Int J Mol Sci. 2020 Jun. PubMed PMID: 32580529
-
Kathleen G. Beavis et al.
Evaluation of the EUROIMMUN Anti-SARS-CoV-2 ELISA Assay for detection of IgA and IgG antibodies
J Clin Virol. 2020 Aug. PubMed PMID: 32485620
-
AJ Jääskeläinen et al.
Performance of six SARS-CoV-2 immunoassays in comparison with microneutralization
J Clin Virol. 2020 Aug. PubMed PMID: 32563180
-
Baoqing Sun et al.
Kinetics of SARS-CoV-2 Specific IgM and IgG Responses in COVID-19 Patients
Emerg Microbes Infect. 2020 Dec. PubMed PMID: 32357808
-
Feng-Cai Zhu et al.
Safety, tolerability, and immunogenicity of a recombinant adenovirus type-5 vectored COVID-19 vaccine: a dose-escalation, open-label, non-randomised, first-in-human trial
Lancet. 2020 13-19 June. PubMed PMID: 32450106
-
Andrea P Espejo et al.
Review of Current Advances in Serologic Testing for COVID-19
Am J Clin Pathol. 2020 Jun 25. PubMed PMID: 32583852
-
Jonathan J Deeks et al.
Antibody tests for identification of current and past infection with SARS-CoV-2
Cochrane Database Syst Rev. 2020 Jun 25. PubMed PMID: 32584464
-
Ahidjo Ayouba et al.
Multiplex detection and dynamics of IgG antibodies to SARS-CoV2 and the highly pathogenic human
coronaviruses SARS-CoV and MERS-CoV
J Clin Virol. 2020 Jun 22. PubMed PMID: 32623350
-
Wan Ni Chia et al.
Serological Differentiation Between COVID-19 and SARS Infections
Emerg Microbes Infect. 2020 Jun 12. PubMed PMID: 32529906
-
Lakshmanane Premkumar et al.
The receptor binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients
Sci Immunol. 2020 Jun 11. PubMed PMID: 32527802
Nat Med. 2020 Jun 5. PubMed PMID: 32504052
-
Florian Krammer et al.
Serology assays to manage COVID-19
Science. 2020 Jun 5. PubMed PMID: 32414781
Clin Transl Med . 2020 Jun 5. PubMed PMID: 32508026
-
Roshan J. D'Cruz et al.
Laboratory Testing Methods for Novel Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2)
Front Cell Dev Biol. 2020 Jun 4. PubMed PMID: 32582718
Cell Rep. 2020 Jun 2. PubMed PMID: 32426212
-
Chek Meng Poh et al.
Two Linear Epitopes on the SARS-CoV-2 Spike Protein That Elicit Neutralising Antibodies in COVID-19 Patients
Nat Commun . 2020 Jun 1. PubMed PMID: 32483236
-
Daniel Stadlbauer et al.
SARS‐CoV‐2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup
Curr Protoc Microbiol. 2020 Jun. PubMed PMID: 32302069
-
Qiang Wang et al.
A Method to Prevent SARS-CoV-2 IgM False Positives in Gold Immunochromatography and Enzyme-Linked Immunosorbent Assays
J Clin Microbiol. 2020 Jun. PubMed PMID: 32277023
-
Ying Yan et al.
Laboratory testing of SARS‐CoV, MERS‐CoV, and SARS‐CoV‐2 (2019‐nCoV): Current status, challenges, and countermeasures
Rev Med Virol. 2020 May. PubMed PMID: 32302058
-
Huan Ma et al.
Serum IgA, IgM, and IgG responses in COVID-19
Cell Mol Immunol. 2020 May 28. PubMed PMID: 32467617
-
J Van Elslande et al.
Diagnostic Performance of Seven Rapid IgG/IgM Antibody Tests and the Euroimmun IgA/IgG ELISA in COVID-19 Patients
Clin Microbiol Infect. 2020 May. PubMed PMID: 32473953
-
Huan Ma et al.
Serum IgA, IgM, and IgG responses in COVID-19
Cell Mol Immunol. 2020 May 28. PubMed PMID: 32467617
-
Nadin Younes et al.
Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2
Viruses. 2020 May 26. PubMed PMID: 32466458
-
Wanbing Liu et al.
Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies Against SARS-CoV-2
J Clin Microbiol . 2020 May 26. PubMed PMID: 32229605
-
Ling Zhong et al.
Detection of serum IgM and IgG for COVID-19 diagnosis
Sci China Life Sci. 2020 May. PubMed PMID: 32270436
-
Alba Grifoni et al.
Targets of T Cell Responses to SARS-CoV-2 Coronavirus in Humans with COVID-19 Disease and Unexposed Individuals
Cell. 2020 May 20. PubMed PMID: 32473127
-
Trevor R F Smith et al.
Immunogenicity of a DNA Vaccine Candidate for COVID-19
Nat Commun. 2020 May 20. PubMed PMID: 32433465
-
Marie Tré-Hardy et al.
The role of serology for COVID-19 control: Population, kinetics and test performance do matter
J Infect. 2020 May 15. PubMed PMID: 32417311
-
Johannes Korth et al.
SARS-CoV-2-specific antibody detection in healthcare workers in Germany with direct contact to COVID-19 patient
J Clin Virol. 2020 Jul (Published online 2020 May 13.) PubMed PMID: 32434708
-
Fatima Amanat et al.
A Serological Assay to Detect SARS-CoV-2 Seroconversion in Humans
Nat Med. 2020 May 12. PubMed PMID: 32511441 -
Jiajia Xie et al.
Characteristics of patients with coronavirus disease (COVID‐19) confirmed using an IgM‐IgG antibody test
J Med Virol. 2020 May 7. PubMed PMID: 32330303
-
Anne J Jääskeläinen et al.
Evaluation of commercial and automated SARS-CoV-2 IgG and IgA ELISAs using coronavirus disease (COVID-19) patient samples
Euro Surveill. 2020 May 7. PubMed PMID: 32400364
-
Rongqing Zhao et al.
Early Detection of SARS-CoV-2 Antibodies in COVID-19 Patients as a Serologic Marker of Infection
Clin Infect Dis. 2020 May. PubMed PMID: 32357209 -
Hongzhou Lu et al.
An Evolving Approach to the Laboratory Assessment of COVID-19
J Med Virol . 2020 Apr 29. PubMed PMID: 32347966 -
Quan-Xin Long et al.
Antibody Responses to SARS-CoV-2 in Patients With COVID-19
Nat Med. 2020 Apr 29. PubMed PMID: 32350462 -
Kai Duan et al.
Effectiveness of convalescent plasma therapy in severe COVID-19 patients
Proc Natl Acad Sci U S A. 2020 Apr 28. PubMed PMID: 32253318
-
Juanjuan Zhao et al.
Antibody Responses to SARS-CoV-2 in Patients of Novel Coronavirus Disease 2019
Cin Infect Dis. 2020 Mar 28. PubMed PMID: 32221519
J Med Virol. 2020 Apr 28. PubMed PMID: 32343415
-
Chenguang Shen et al.
Treatment of 5 Critically Ill Patients With COVID-19 With Convalescent Plasma
JAMA. 2020 Apr 28. PubMed PMID: 32219428
-
Ranawaka APM Perera et al.
Serological assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), March 2020
Euro Surveill. 2020 Apr 23. PubMed PMID: 32347204
-
Andrea Padoan et al.
IgA-Ab Response to Spike Glycoprotein of SARS-CoV-2 in Patients With COVID-19: A Longitudinal Study
Clin Chim Acta. 2020 Aug. PubMed PMID: 32343948
-
Nisreen M A Okba et al.
Emerg Infect Dis. 2020 Apr 8. PubMed PMID: 32267220
参考文献
1.)平松 啓一, 標準微生物学, 第 11 版, 医学書院 (2012)
2.)Anu Haveri , “Serological and molecular findings during SARS-CoV-2 infection: the first case study in Finland,” January to February 2020 , Eurosurveillance. Volume 25, Issue 11, 19, Mar 2020.
3.)Wanbing Liu, “Evaluation of Nucleocapsid and Spike Protein-based ELISAs for detecting antibodies against SARS-CoV-2”, J Clin Microbiol. 2020 Mar 30.
4.)Li Guo, “Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19)”, Clin Infect Dis. 2020 Mar 21.
5.)Juanjuan Zhao, “Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019”, Clin Infect Dis. 2020 Mar 21.