
COVID-19 Human IgM IgG ELISAキット(Nucleocapsid Protein)
マニュアル
SDS
フライヤー
製品の主な仕様
測定方法: 間接 ELISA 法
測定波長: 450 nm
測定試料: 血清
交差性: Human
保管温度: 4°C保管
品質保証期限: 本品の有効期限は製造後 12 カ月です。
プレートへの固相化抗原: SARS-CoV-2 ヌクレオカプシドタンパク(全長, 大腸菌発現系)
キット内容
1. SARS-CoV-2 ヌクレオカプシドタンパク固相化プレート
2. HRP 標識抗ヒト IgM 抗体
3. HRP 標識抗ヒト IgG 抗体
4. 洗浄液(10× PBST)
5. ウシ血清アルブミン(Diluent 用)
6. Diluent 用バッファー (10×)
7. R-1:発色基質 (TMB)
8. R-2:反応停止液 (1 mol/L 塩酸)
9. マイクロプレートシール
×2
20 μL×1
20 μL×1
100 mL×1
×1
50 mL×1
22 mL×1
22 mL×1
×2
測定原理
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固相化プレート上の リコンビナント SARS-CoV-2 ヌクレオカプシドタンパク と、これを認識する試料中の抗体(以後、抗ヌクレオカプシドタンパク抗体と略称)を反応させる。
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反応後、試料を洗浄操作により除去する。
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固相化プレート上の 抗ヌクレオカプシドタンパク抗体と、HRP (Horseradish peroxidase) 標識抗ヒト IgM / IgG 抗体を反応させる。
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余剰の HRP 標識抗ヒト IgM / IgG 抗体を洗浄操作により除去する。発色基質を加え、吸光度を測定する。

測定の意義
本キットは、COVID-19 の原因ウイルスである、SARS-CoV-2 のヌクレオカプシドタンパクを認識するヒトIgM 抗体とヒト IgG 抗体を検出するための研究用 ELISA キットです。
ウイルスの検出には、大きく分けて二種類のアプローチ法があり、PCR 法などのウイルス自体を検出する方法と、感染時に発現する免疫反応(IgG, IgM, IgA)を検出する方法があります。一般的 な感染症において、IgM は感染初期に生成される抗体で、症状の緩和とともに消失します。IgG は IgM に遅れて産生が始まり、治癒後も数カ月にわたって産生され続けます。これにより次に感染した際の症状が軽くなったり、発症を防いだりすることが知られています。
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