COVID-19 Human IgM IgG ELISAキット(Nucleocapsid Protein)

​SDS

測定方法:　　　間接 ELISA 法

測定波長:　　　450 nm

測定試料:　　　血清

交差性:　　　　Human

保管温度:　　　4°C保管

品質保証期限:   本品の有効期限は製造後 12 カ月です。

プレートへの固相化抗原:　SARS-CoV-2 ヌクレオカプシドタンパク（全長, 大腸菌発現系）

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キット内容

1. SARS-CoV-2 ヌクレオカプシドタンパク固相化プレート

2. HRP 標識抗ヒト IgM 抗体

3. HRP 標識抗ヒト IgG 抗体

4. 洗浄液(10× PBST)

5. ウシ血清アルブミン（Diluent 用）

6. Diluent 用バッファー (10×)

7. R-1:発色基質 (TMB)

8. R-2:反応停止液 (1 mol/L 塩酸)

9. マイクロプレートシール

×2

20 μL×1

20 μL×1

100 mL×1

×1

50 mL×1

22 mL×1

22 mL×1

×2

測定原理

1. 固相化プレート上の リコンビナント SARS-CoV-2 ヌクレオカプシドタンパク と、これを認識する試料中の抗体(以後、抗ヌクレオカプシドタンパク抗体と略称)を反応させる。

2. 反応後、試料を洗浄操作により除去する。

3. 固相化プレート上の 抗ヌクレオカプシドタンパク抗体と、HRP (Horseradish peroxidase) 標識抗ヒト IgM / IgG 抗体を反応させる。

4. 余剰の HRP 標識抗ヒト IgM / IgG 抗体を洗浄操作により除去する。発色基質を加え、吸光度を測定する。

　本キットは、COVID-19 の原因ウイルスである、SARS-CoV-2 のヌクレオカプシドタンパクを認識するヒトIgM 抗体とヒト IgG 抗体を検出するための研究用 ELISA キットです。 

　ウイルスの検出には、大きく分けて二種類のアプローチ法があり、PCR 法などのウイルス自体を検出する方法と、感染時に発現する免疫反応(IgG, IgM, IgA)を検出する方法があります。一般的 な感染症において、IgM は感染初期に生成される抗体で、症状の緩和とともに消失します。IgG は IgM に遅れて産生が始まり、治癒後も数カ月にわたって産生され続けます。これにより次に感染した際の症状が軽くなったり、発症を防いだりすることが知られています。

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本キット使用文献

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  Antibody Responses to SARS-CoV-2 in Patients of Novel Coronavirus Disease 2019
  Cin Infect Dis. 2020 Mar 28. PubMed PMID: 32221519

• Li Guo et al.
  Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19)
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参考文献

1.)平松　啓一, 標準微生物学, 第 11 版, 医学書院 (2012)

2.)Anu Haveri , “Serological and molecular findings during SARS-CoV-2 infection: the first case study in Finland,” January to February 2020 , Eurosurveillance.  Volume 25, Issue 11, 19, Mar 2020.

3.)Wanbing Liu, “Evaluation of Nucleocapsid and Spike Protein-based ELISAs for detecting antibodies against SARS-CoV-2”, J Clin Microbiol. 2020 Mar 30.

4.)Li Guo, “Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19)”, Clin Infect Dis. 2020 Mar 21.

5.)Juanjuan Zhao, “Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019”, Clin Infect Dis. 2020 Mar 21.

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